Xian Institute of Optics and Precision Mechanics Makes New Progress in Label-Free 3D Microscopic Imaging

Xi'an, August 2 — The team led by Researcher Yao Baoli from the Transient Optics Laboratory at the Xi'an Institute of Optics and Precision Mechanics has recently made progress in labelfree 3D microscopic imaging. This achievement centers on optical diffraction tomography and aims to enhance the ability to observe the threedimensional structures of transparent or semitransparent samples. The research team proposed an LEDODT method that combines programmable LED array illumination with a quantitative phase camera, and implemented the modification on the basis of a standard commercial microscope. Through rapid multiangle illumination, the system acquires the complex amplitude information of the sample, and then uses relevant reconstruction methods to recover the threedimensional refractive index distribution. According to the introduction, the compact system built by the team consists of a 15×15 programmable LED array and a Qcamera, and can complete image acquisition at 225 illumination angles within 2.7 seconds. This technology features a simpler structure and more convenient operation, and is expected to provide a new tool for the threedimensional quantitative imaging of cells, biological tissues, and material samples.